Measuring the 50% Haemolytic Complement (CH<sub>50</sub>) Activity of Serum
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چکیده
منابع مشابه
Measuring the 50% haemolytic complement (CH50) activity of serum.
The complement system is a group of proteins that when activated lead to target cell lysis and facilitates phagocytosis through opsonisation. Individual complement components can be quantified however this does not provide any information as to the activity of the pathway. The CH50 is a screening assay for the activation of the classical complement pathway (Fig 1) and it is sensitive to the red...
متن کاملStudies on the haemolytic complement of the dromedary camel (Camelus dromedarius). I. Classical pathway haemolytic activity in serum.
Classical pathway haemolytic complement (CPHC) of the dromedary was assayed under standardised conditions. A total of 14 indicator systems of red blood cells (RBC) and haemolysins were investigated. Highest CH50 titre was obtained with rabbit RBC sensitised with goat haemolysin. Among the factors investigated were: ionic strength, Mg2+, Ca2+, ethylenediaminetetraacetic acid (EDTA) concentration...
متن کاملMeasurement of total haemolytic complement activity in body fluids.
The measurement of total haemolytic complement is of diagnostic value in the rheumatic diseases. A method which is easier to perform than that of Kabat and Mayer (Experimental Immunochemistry, 2nd ed., Thomas, Springfield, Ill.) is described. A unit based on the mean value of the complement activities of normal sera is proposed to replace CH50 units. Other steps by which standardisation of the ...
متن کاملMeasurement of total haemolytic complement activity in body fluids
The measurement of total haemolytic complement is of diagnostic value in the rheumatic diseases. A method which is easier to perform than that of Kabat and Mayer (Experimental Immunochemistry, 2nd ed., Thomas, Springfield, Ill.) is described. A unit based on the mean value of the complement activities of normal sera is proposed to replace CH50 units. Other steps by which standardization of the ...
متن کاملThe relationship between the minimal and the 50% haemolytic dose in complement titrations.
The 50% spectrophotometric complement titration end-point (C'H50) was found to be more reproducible than the 100% end-point. The relationship of the 100% end-point (MHD) to the (C'H50) was 1:2, 5. The use of thrombin tubes induced clotting within 2 min and enabled the process of complement preparation to be completed well within 1 hour.
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2010
ISSN: 1940-087X
DOI: 10.3791/1923